Journal: eLife

Article Title: Hypoxia-inducible factor 1 signaling drives placental aging and can provoke preterm labor

doi: 10.7554/eLife.85597

Figure Lengend Snippet: Weighted gene correlation network analysis (WGCNA) yielded 20 gene clusters. Functional pathways overrepresented in clusters found to increase (blue) and decrease (turquoise) across gestation highlight enhanced cellular senescence, increased HIF-1 signaling, and decreased mitochondrial synthesis and respiration late in pregnancy ( A ). mRNA expression of senescence marker Glb1 peaks at e17.5 (B; one-way ANOVA p=0.0048). HIF-1 protein abundance is higher at e17.5 versus e13.5 and e15.5 (C; one-way ANOVA p=0.019), as is expression of HIF-1 targets Hk2 and Slc2a1 (D; two-way ANOVA p<0.0001 for gestational age factor). (See for analysis of gene expression changes across timepoints by placental sex.) Mitochondrial abundance, reflected by COX IV protein, decreases with gestational age (E, one-way ANOVA p=0.0064), and mitochondrial DNA lesion rate peaks at e17.5 in the regions of the D-loop (one-way ANOVA p=0.0001), COII/ATPase6 (p=0.0027), and ND5 (p=0.036) ( F ). (B–F) Each data point represents a biological replicate (e.g. RNA, protein, or DNA extracted from an individual placenta, in turn collected from one of 2–4 pregnant dams per group). Data normalized to mean at e13.5. See for uncropped blots. Figure 2—source data 1. Uncropped, unedited blots from 2c (left) and 2e (right).

Article Snippet: MT-COX2 , Hs02596865_g1 , TaqMan.

Techniques: Functional Assay, Expressing, Marker, Quantitative Proteomics, Gene Expression

Journal: eLife

Article Title: Hypoxia-inducible factor 1 signaling drives placental aging and can provoke preterm labor

doi: 10.7554/eLife.85597

Figure Lengend Snippet: mRNA expression of senescence marker GLB1 and HIF-1 targets HK2 and SLC2A1 trends higher in placentas from >39-week cohort vs <35-week cohort (A; two-way ANOVA gestational age factor p=0.057). Mitochondrial abundance, reflected by mitochondrial genes ATP6 and COX2 (B; two-way ANOVA gestational age factor p=0.042) and COX IV protein (C; p=0.0036) decreases with advancing gestational age. Each data point represents a biological replicate (RNA or protein isolated from an individual placenta). Data normalized to mean in <35-week group. See for uncropped blots. Figure 3—source data 1. Uncropped, unedited blots from .

Article Snippet: MT-COX2 , Hs02596865_g1 , TaqMan.

Techniques: Expressing, Marker, Isolation

Journal: eLife

Article Title: Hypoxia-inducible factor 1 signaling drives placental aging and can provoke preterm labor

doi: 10.7554/eLife.85597

Figure Lengend Snippet: HIF-1 is detected in cultured trophoblasts exposed to CoCl 2 ( A ). After 48 hr of CoCl 2 exposure, trophoblasts exhibit decreased mitochondrial abundance reflected by Cox2 mRNA expression levels (B; p=0.014) and COX IV protein levels (C; p=0.0047). Senescence marker Glb1 is increased (D; p=0.038) and senescence-associated beta galactosidase (SA-βGal) accumulation is noted by X-gal assay (E; p=0.012). Each data point represents a technical replicate (e.g. protein, RNA, or β-Gal measured from an individual well of cells grown in treated or control condition). Data normalized to mean of control treatment group. See for uncropped blots. Figure 4—source data 1. Uncropped, unedited blots from 4a (left) and 4c (right).

Article Snippet: MT-COX2 , Hs02596865_g1 , TaqMan.

Techniques: Cell Culture, Expressing, Marker, Control

Journal: Antioxidants

Article Title: Biological Implications of Differential Expression of Mitochondrial-Shaping Proteins in Parkinson’s Disease

doi: 10.3390/antiox7010001

Figure Lengend Snippet: List of mitochondrial-shaping proteins.

Article Snippet: IMMT, HMP, MIC60, MINOS2, PIG4, PIG52 , MICOS Complex subunit MIC60(Cell proliferation-inducing gene 4/52 protein) (Inner mitochondrial membrane protein) (Mitofilin) (p87/89) , Component of the MICOS Complex, a large protein Complex of the inner mitochondrial membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane , IMM , Cristae shape , [ , , , , ] .

Techniques: Translocation Assay, Cell Differentiation, Inhibition, Activity Assay, Derivative Assay, DNA Synthesis, Transformation Assay, Permeability, Functional Assay, Transduction

Journal: Antioxidants

Article Title: Biological Implications of Differential Expression of Mitochondrial-Shaping Proteins in Parkinson’s Disease

doi: 10.3390/antiox7010001

Figure Lengend Snippet: List of binding partners of the mitochondrial-shaping proteins altered in Parkinson’s disease ( Table 2 ) that are found in the list of proteins modified in the context of Parkinson’s disease ( Supplementary Table S1 ).

Article Snippet: IMMT, HMP, MIC60, MINOS2, PIG4, PIG52 , MICOS Complex subunit MIC60(Cell proliferation-inducing gene 4/52 protein) (Inner mitochondrial membrane protein) (Mitofilin) (p87/89) , Component of the MICOS Complex, a large protein Complex of the inner mitochondrial membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane , IMM , Cristae shape , [ , , , , ] .

Techniques: Binding Assay, Modification, Migration, Activity Assay, Transformation Assay, Histone Deacetylase Assay

Journal: Stem Cells International

Article Title: BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D In Vitro Models

doi: 10.1155/2022/4910399

Figure Lengend Snippet: Relative levels of gene expression of 2D-cultured MG-63 cells, hADSCs and hDPSCs, for 2 and 3 weeks with proliferation medium or commercial MesenCult osteogenic differentiation medium of (a) RUNX2, (b) BGLAP, (c) IBSP, (d) SPP1, (e) CSF1, (f) CSF2, and (g) MT-CO1. Samples cultured with proliferation media were taken as relative control. Mean ± SD are shown, with statistical significance at ∗ p ≤ 0.05 with respect to the control group.

Article Snippet: For amplifications, the following predesigned Assays on Demand (Life Technologies; Spain) were used: BGLAP (Hs01587813_g1), CSF1 (Hs00174164_m1), CSF2 (Hs00929873_m1), IBSP (Hs00913377_m1), MT-CO1 (Hs02596864_g1), RUNX2 (Hs01047973_m1), and SPP1 (Hs00959010_m1).

Techniques: Gene Expression, Cell Culture, Control

Journal: Stem Cells International

Article Title: BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D In Vitro Models

doi: 10.1155/2022/4910399

Figure Lengend Snippet: Relative levels of gene expression of 2D-cultured MG-63 cells, hADSCs and hDPSCs, for 2 and 3 weeks with proliferation medium or BMP2-supplemented osteogenic differentiation medium of (a) RUNX2, (b) BGLAP, (c) IBSP, (d) SPP1, (e) CSF1, (f) CSF2, and (g) MT-CO1. Samples cultured with proliferation media were taken as relative control. Mean ± SD are shown, with statistical significance at ∗ p ≤ 0.05 with respect to the control group.

Article Snippet: For amplifications, the following predesigned Assays on Demand (Life Technologies; Spain) were used: BGLAP (Hs01587813_g1), CSF1 (Hs00174164_m1), CSF2 (Hs00929873_m1), IBSP (Hs00913377_m1), MT-CO1 (Hs02596864_g1), RUNX2 (Hs01047973_m1), and SPP1 (Hs00959010_m1).

Techniques: Gene Expression, Cell Culture, Control